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bacterial rna miniprep kit  (Zymo Research)


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    Structured Review

    Zymo Research bacterial rna miniprep kit
    Bacterial Rna Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quick+rna+fungal/Quick-RNA+Fungal%2FBacterial+Miniprep+Kit/pm42049898-356-7-11
    Average 97 stars, based on 313 article reviews
    bacterial rna miniprep kit - by Bioz Stars, 2026-09
    97/100 stars

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    Isolation:

    Article Title: Extracellular Metabolite Profiling in CO2-Fixing Bacterium Rhodobacter sphaeroides Under Autotrophic Conditions
    Article Snippet: .. Harvested cells were washed twice with DNase/RNasefree distilled water, and total RNA was isolated according to the manufacturer’s protocol using the Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). .. Genomic DNA contamination was removed by treatment with RNase-free DNase I. Extracted RNA samples were quantified using a NanoDrop 2000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA).

    Article Title: Extracellular Metabolite Profiling in CO 2 -Fixing Bacterium Rhodobacter sphaeroides Under Autotrophic Conditions
    Article Snippet: .. Harvested cells were washed twice with DNase/RNase-free distilled water, and total RNA was isolated according to the manufacturer’s protocol using the Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). .. Genomic DNA contamination was removed by treatment with RNase-free DNase I. Extracted RNA samples were quantified using a NanoDrop 2000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA).

    Article Title: Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens
    Article Snippet: When the main cultures reached an OD 600 nm of 0.5, 3 mL of the cell suspensions were mixed with 6 mL of RNAprotect® Bacteria Reagent (QIAGEN, Germany) by vortexing and incubated for 5–15 min at RT to stabilize RNA transcripts. .. Subsequently, stabilized cultures were centrifuged for 10 min at 5000 g. Most of the supernatant was decanted, the pellet resuspended in the remaining liquid and centrifuged again for 2 min at 5000 g. For RNA isolation, the Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research, Freiburg, Germany) including DNase I treatment was used according to the manufacturer’s protocol. ..

    Incubation:

    Article Title: Role of the transcription factor Wor2 in biofilm formation of Candidozyma auris .
    Article Snippet: .. After 24 h of incubation at 37°C, biofilms were gently scraped, and the biomass was collected by centrifugation at 2,500 rpm for 5 min. RNA extraction was performed using the Quick-RNA Fungal/Bacterial Miniprep Kit (ZymoResearch, Lucerna-Chem AG). .. RNA was purified using a DNA-free kit (Thermo Fisher), and cDNA was synthesized (PrimeScript 1st strand cDNA synthesis kit, Takara Bio).

    Centrifugation:

    Article Title: Role of the transcription factor Wor2 in biofilm formation of Candidozyma auris .
    Article Snippet: .. After 24 h of incubation at 37°C, biofilms were gently scraped, and the biomass was collected by centrifugation at 2,500 rpm for 5 min. RNA extraction was performed using the Quick-RNA Fungal/Bacterial Miniprep Kit (ZymoResearch, Lucerna-Chem AG). .. RNA was purified using a DNA-free kit (Thermo Fisher), and cDNA was synthesized (PrimeScript 1st strand cDNA synthesis kit, Takara Bio).

    Article Title: Fungal extracellular vesicles mediate conserved cross-species communication and immunomodulation
    Article Snippet: .. Cells were then separated from the culture medium by centrifugation at 8,000 × g for 15 min at 4°C and subjected to RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research). .. The isolated RNA was quantified using a NanoDrop One spectrophotometer (Thermo) and subsequently used to evaluate the expression levels of LAC1 , URE1, and CAP59 by qPCR , with results compared to the control culture ( C. neoformans cap67 Δ without EV addition).

    RNA Extraction:

    Article Title: Role of the transcription factor Wor2 in biofilm formation of Candidozyma auris .
    Article Snippet: .. After 24 h of incubation at 37°C, biofilms were gently scraped, and the biomass was collected by centrifugation at 2,500 rpm for 5 min. RNA extraction was performed using the Quick-RNA Fungal/Bacterial Miniprep Kit (ZymoResearch, Lucerna-Chem AG). .. RNA was purified using a DNA-free kit (Thermo Fisher), and cDNA was synthesized (PrimeScript 1st strand cDNA synthesis kit, Takara Bio).

    Article Title: Fungal extracellular vesicles mediate conserved cross-species communication and immunomodulation
    Article Snippet: .. Cells were then separated from the culture medium by centrifugation at 8,000 × g for 15 min at 4°C and subjected to RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research). .. The isolated RNA was quantified using a NanoDrop One spectrophotometer (Thermo) and subsequently used to evaluate the expression levels of LAC1 , URE1, and CAP59 by qPCR , with results compared to the control culture ( C. neoformans cap67 Δ without EV addition).



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